Voigt spectral profiles in two-photon resonance fluorescence

Simultaneous two-photon spectral and lifetime fluorescence microscopy.

When a fluorescence photon is emitted from a molecule within a living cell it carries a signature that can potentially identify the molecule and provide information on the microenvironment in which it resides, thereby providing insights into the physiology of the cell. To unambiguously identify fluorescent probes and monitor their physiological environment within living specimens by their fluor...

Two-photon Fluorescence Endomicroscopy

Photon statistics of resonance fluorescence in the limit of separated spectral lines.

We have studied the statistics of fluorescent photons emitted by a two-state atom in a laser beam in the limit where either the detuning or the Rabi frequency is large. For this case, the spectrum of resonance fluorescence has three separated lines. We have obtained closed form expressions for the conditional probability density for the emission of the nth photon and for the probability for emi...

Two-photon excited hemoglobin fluorescence

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. Thi...

Two-photon Fluorescence Light Microscopy

The invention of two-photon fluorescence light microscopy by Denk, Webb and co-workers (Denk et al., 1990) revolutionized three-dimensional (3D) in vivo imaging of cells and tissues. In 1931, the theoretical basis of twophoton excitation was established by Maria GöppertMayer, and this photophysical effect was verified experimentally by Kaiser and Garret in 1963. Two-photon excitation is a fluor...